Is Acetonitrile miscible with n-Hexane?
Compiled by Hemant RawatLast reviewed July 2026How we verify
Immiscible
No — Acetonitrile and n-Hexane are immiscible. They do not mix and instead settle into two separate layers.
Why
The mismatch is polarity. Acetonitrile is polar aprotic (polarity index 5.8) while n-Hexane is nonpolar (polarity index 0.1). "Like dissolves like": a strongly polar solvent and a non-polar one have too little in common to form one phase, so they separate — the same reason oil and water don't mix.
What it means for your method
In practice, you can't use these two directly in one mobile phase. To bridge them, add a mutually-miscible co-solvent (e.g. an alcohol or acetone) so the mixture stays one phase.
Check any other pair on the interactive miscibility chart, or see full properties for Acetonitrile and n-Hexane.
General guidance for method development — miscibility varies with temperature, water content and proportions. Confirm critical mixtures at your working conditions. See ourmethodology.